Bacterial expression work is routinely carried out with a number of popular expression vector and fusion protein systems as well as secretion systems (allowing periplasmic folding of secreted proteins such as antibody fragments). The exact choice depends on the project requirements and will be discussed and incorporated into the project proposal. Success of an E. coli expression experiment is enhanced by optimizing variables beyond the expression vector system, such as the choice of bacterial expression strain, use of helper plasmids for tRNA or chaperone supplementation, induction strength and culture conditions (medium, temperature etc.).
A range of expression scales is available. Fully scaled-up expression is carried out by 20 liter batch / fed-batch cultures in a modern fermenter system. High cell densities in fermentation cultures allow yields between several hundred milligram and several grams of recombinant protein.
Fermentation of other microbial hosts is under development (please enquire for details).
