Purification

The protein specialists at DIARECT have years of experience purifying proteins from a wide variety of sources. This includes the purification of endogenous proteins from virtually any source as well as the purification of heterologously expressed proteins from cells and media used in typical expression systems.

The backbone of our purification strategies are state-of-the-art Äkta FPLC systems. In addition, high-speed and continuous-flow centrifuges are available as well as a hollow fiber filtration system for easy and efficient handling of large volumes. These systems allow us to process starting material for the purification of microgram to gram amounts of protein.

Purification Features

  • Range from microgram to gram amounts
  • Purification from any supplied starting material (S1)
  • Published, customer-supplied or custom-designed purification strategies
  • All standard chromatography modes can be used on dedicated columns
  • Endotoxin removal
  • Continuous monitoring of purification process by SDS-PAGE and Western Blot analysis