DNA Topoisomerase I (Scl-70; truncated)

Cat. No.	Available in
14500	0.1 mg
14501	1 mg

Download the Antigen Specification in PDF format

Download MSDS information in PDF format

DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.

In human tissues the DNA topoisomerase I is initially synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the Scl-70 antigen has derived its name.

DNA topoisomerase I (Scl-70) antigens from DIARECT are produced in the baculovirus / insect cell expression system. To allow optimal assay development DIARECT offers two distinct DNA topoisomerase I antigens: one is the full-length 100-kDa enzyme precursor (follow this link to the page with the details of the full-length form), the other one is a truncated form resembling the processed 70-kDa form of the antigen (details described on this page).