dsDNA-mAb33

Cat. No.Available inproduct_image_51
33300Trial size
33301Standard size
 

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Patient sera are traditionally used for standardizing diagnostic autoantibody assays. Well-known problems arise from limited supply, multispecificity and variability of titers and reactivities. Monoclonal or recombinant antibody approaches have not yet provided replacement standardization reagents, due to the difficulties in generating and producing suitable antibodies.

In an exceptional study, Winkler et al. (1991) have established from peripheral B lymphocytes of SLE patients a panel of human heterohybridoma cell lines which produce high-affinity monoclonal autoantibodies against double-stranded DNA (dsDNA). The properties of these monoclonal autoantibodies have been studied in detail (Simon et al., 1996), and several of the antibodies can be used for standardization of different types of anti-dsDNA autoantibody assays:

  • dsDNA-mAb33, when tested in ELISA-based devices, shows an extremely good correlation to the International WHO standard preparation for anti-dsDNA antibodies (Wo/80) and can adequately replace the traditional polyclonal standards.

  • Its companion antibody dsDNA-mAb32 (cat. no. 33200) has shown excellent applicability in Farr and Crithidia-type assays.

For uses as standardization reagent, dsDNA-mAb33 is available from DIARECT as purified and stabilized protein.