gp210
| Cat. No. | Available in |  |
| 19000 | 0.1 mg | |
| 19001 | 1.0 mg | |
Data Sheet (english)Diseases:
Primary Biliary Cirrhosis
The nuclear envelope separates cytoplasm from the nuclear compartment in eukaryotic cells. It is composed of a double membrane, pore complexes, and the nuclear lamina. Among the best characterized pore complex proteins are two distinct glycoproteins with molecular masses of 62 and 210 kDa. The latter, termed gp210, is an integral membrane protein that carries high mannose-type oligosaccharides in its large luminal section. gp210 is believed to be involved in the formation of nuclear pores and the attachment of pore complex constituents to the nuclear membrane.
Although M2 autoantibodies can be qualified as the most characteristic serological marker for PBC, there are a number of antibodies against nuclear components present in sera of up to 50% of these patients. A perinuclear staining pattern by indirect immunoflurescence is indicative of the reactivity of the sera with the components of the nuclear envelope. Antibodies directed against gp210 are found in about 25 % of patients with AMA/M2-positive PBC and up to 50% of those with M2-negative PBC. The disease specificity of anti-gp210 is close to 100%. In contrast to M2 antibodies, gp210 antibodies have prognostic significance and are thought to correlate with progression toward end-stage liver disease. The relevant epitopes are located in the short cytoplasmic portion of the gp210 protein.
DIARECT's recombinant gp210 antigen has been optimally designed for assay performance and high-level expression of the diagnostically relevant epitopes in the baculovirus/insect cell system.