Intrinsic Factor

Cat. No.	Available in
16700	0.1 mg
16701	1 mg

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Circulating antibodies against gastric intrinsic factor (IF) are highly specific and of high diagnostic relevance for pernicious anemia. Two types of clinically important autoantibodies can be measured: (i) so-called blocking antibodies which prevent the binding of vitamin B₁₂ to the IF molecule; they are present in >50% of all patients with Biermer anemia; (ii) other types of antibodies to IF may interfere with the binding of the IF-Vit B₁₂ complex to the ileal receptor and are less common and sensitive.

Intrinsic factor is produced by DIARECT in the baculovirus / Sf9 insect cell expression system. This high-quality antigen is secreted from the insect cell along the endosomal pathway via Golgi glycosylation and passes into the culture medium. Endogenous bound cobalamin/vitamin B₁₂ is depleted during the purification process, exposing all relevant epitopes for autoantibody binding on the recombinant intrinsic factor.