Mi-2

Cat. No.	Available in
18100	0.1 mg
18101	1.0 mg

Specification Sheet

MSDS

The Mi-2ß autoantigen (MW 221.3 kDa) belongs to a family of so-called chromodomain helicase-DNA-binding proteins; several isoforms of Mi-2 exist with molecular weights between 210 and 240 kDa. Biochemically, Mi-2ß is a component of the nucleosome remodeling and deacetylase (NuRD) complex, which seems to be involved in transcription regulation.

Autoantibodies targeting the Mi-2 nuclear antigen are a serologic feature of idiopathic inflammatory myopathies (IIM). In IIM Mi-2 antibodies are characterized by diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. Furthermore, anti-Mi-2 antibodies are strongly associated with dermatomyositis (frequency up to 31%) and have a very high positive predictive value for such a disease subset. Anti-Mi-2 are the only defined myositis-specific autoantibodies clearly directed to a nuclear target. Another rather exceptional feature of Mi-2 antibodies relates to their frequency in children, which is comparable to that in adults.

Previously, only several recombinant Mi-2ß subfragments expressed in E.coli have been used in diagnostic applications. Recombinant, human Mi-2ß from DIARECT is a full-length, 220-kDa autoantigen, eukaryotically expressed in baculovirus-infected insect cells. DIARECT is the first commercial supplier offering a recombinant full-length Mi-2 antigen.