Tissue Transglutaminase (tTG; expressed in Baculovirus/Sf9)
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Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease, and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is continuously replacing the traditionally used tTG fraction from guinea pig.
Tissue-type transglutaminase antigens from DIARECT have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations by eliminating variable and ill-defined covalent aggregates of tTG antigen and host-cell proteins.
Two recombinant human tissue transglutaminase products are available from DIARECT, produced in baculovirus / insect cell expression system and E. coli, respectively (follow this link for the page of the E. coli-expressed tTG antigen).