DIARECT
 

DIARECT Newsletter

Summary

Dear Madam or Sir,

As in the previous years, DIARECT will be exhibiting at MEDICA 2015, the world’s largest medical trade show that will take place in Düsseldorf, Germany from November 16 – 19, 2015. At this event, we will introduce new proteins recently added to the product lines autoimmune and infectious disease antigens.  
In addition, we are excited to announce the introduction of a complete new product line - recombinant allergens.
Please feel welcome to visit DIARECT’s team members in hall 1 at booth E19 to find out about the latest products and to receive a hard copy of our new product catalog. To access this catalog online, please follow this link: Catalog.

To schedule personal appointments or to receive further information on our products, please contact us at:

Email   info@diarect.com
Tel.     +49 761 47979-0

Pleasant reading!

DIARECT AG

Introducing a new product line: recombinant allergens

Allergies are nowadays considered to be at epidemic levels. Food allergies are among the major forms of allergies and have been reported in 3 - 8 % of children and 1 - 3 % of adults. A second type of allergies is caused by respiratory allergens (aeroallergens), e.g. grass and tree pollens. Respiratory allergies are considered to be even more prevalent than food allergies. The molecular identification of specific allergens and the subsequent establishment of Component Resolved Diagnosis of allergies allows the detection of and distinction between specific and cross-specific IgE antibodies.

DIARECT is proud to announce the introduction of a complete new product line: recombinant food as well as grass and tree pollen allergens. 

Expansion of autoimmune antigens

Anti-nuclear autoantibodies (ANA), initially detected by indirect immunofluorescence using HEp-2 cells, have been described for various systemic autoimmune rheumatic diseases (SARD) including systemic lupus erythematosus, systemic sclerosis, and sjøgren’s syndrome. Importantly, also healthy individuals are found to be serologically positive for ANA in indirect immunofluorescence analyses due to antibodies against a 70-kDa protein giving rise to the so-called dense fine nuclear speckled (DFS) pattern. This protein termed DFS70 is identical with lens epithelium-derived growth factor/transcription coactivator p75 (LEDGF/p75).

Glutamate decarboxylase (GAD) catalyzes the decarboxylation of glutamate yielding in the production of gamma-aminobutyrate (GABA) and CO2. The pancreatic GAD65 isoform localizing in the proximity of the Golgi apparatus and GABA-containing vesicles is the antigen of autoantibodies identified in the sera of patients diagnosed with insulin-dependent diabetes mellitus (IDDM). IDDM is synonymous with type 1 diabetes mellitus (T1DM) and characterized by the destruction of insulin-synthesizing pancreatic betacells. DIARECT now offers recombinant DFS70 and recombinant GAD65.

Update on infectious disease antigens – new pathogens

Recombinant antigens from three new human pathogens will be available from DIARECT. These pathogens are Babesia microti, Candida albicans and Streptococcus pyogenes the causative agents of Babesiosis, Candidiasis and Scarlet Fever, respectively.

Babesia microti is a protozoan parasite of the phylum Apicomplexa, which is transmitted by ticks of the genus Ixodes and infects and replicates within erythrocytes. In endemic areas of the United States, approximately 20% of these ticks are infected with B. microti. In addition, infection can occur by blood transfusions, and babesiosis is considered the most common blood-transfusion associated disease.
DIARECT produces two B. microti antigens useful for immunological assays. These include a 32-kDa secretory protein, Babesia microti p32, and a cytoplasmic interspersed repeat antigen, Babesia microti IRA, that comprises three distinct blocks of repetitive amino acids.

The yeast Candida albicans is an important commensal pathogen that colonizes the skin and mucosal surfaces of 30 - 70% of all humans. In healthy individuals, C. albicans is controlled by the normal microbial flora, epithelial barriers, and the immune system. In situations when this homeostasis becomes imbalanced, e.g. burns leading to the destruction of epithelial barriers or a compromised immune system, a C. albicans infection called candidiasis can develop. DIARECT produces four recombinant antigens for the serology of C. albicans specific antibodies:

  • Candida albicans Hsp70, a member of the heat shock protein 70 family of molecular chaperones, which localizes to both the cytoplasm and the cell wall
  • Candida albicans enolase, a glycolytic enzyme catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate that is also detected in both the cytoplasm and the cell wall
  • Candida albicans glucan 1,3-beta-glycosidase (Bgl2), a cell wall localized 1,3-beta-gylcosyltransferase contributing to C. albicans’ virulence
  • Candida albicans methionine synthase (Met6), an essential and cobalamin-independent enzyme implicated in the synthesis of methionine 

Streptococcus pyogenes is a Gram-positive, round-shaped bacterium categorized as the so-called group A Streptococcus (GAS). S. pyogenes is considered one of the most common human pathogens that spreads via direct contact and aerosols. It is the causative agent of up to 10 and 30% of all cases of pharyngitis (“Strep Throat”) in adults and children, respectively, as well as Scarlet Fever. Following acute S. pyogenes infections, aseptic sequelae affecting different organ systems have been described. These include acute rheumatic fever, rheumatic heart disease, and acute post-streptococcal glomerulonephritis. S. pyogenes arginine deiminase (arcA) hydrolyzes L-arginine into citrulline and ammonia, and is identical with streptococcal acid glycoprotein (SAGP). S. pyogenes transketolase (tkt) catalyzes two reactions in the pentose phosphate pathway that lead to the conversion of ribulose-5-phosphate to glyceraldehyde-3-phosphate and fructose-6-phosphate. Both proteins are produced by DIARECT.

For questions or additional information please feel free to contact us at:
Tel: +49 761 47979-0
Email: orders@diarect.com

Distributor in North America and Canada

To facilitate the purchase of DIARECT’s products throughout the world, DIARECT concluded distribution agreements with select companies.

If you are located in North America or Canada, DIARECT’s products are exclusively distributed by:

SurModics IVD, Inc.

9924 West 74th Street
Eden Prairie, MN 55344
USA
Tel +1 952 500 7200
Toll-free +1 800 755 7793
Fax +1 952 500 7201
orders@surmodics.com
www.surmodicsivd.com

Distributor in China

To facilitate the purchase of DIARECT’s products throughout the world, DIARECT concluded distribution agreements with select companies.

If you are located in China, DIARECT’s products are distributed by:

Shanghai BioSun Sci&Tech Co., Ltd.
Room K, 5F, Bldg. No.1
825 Zhaojiabang Road
Shanghai 200032
P.R. China
Tel +86 21 54253791
Fax +86 21 64280007
biosun@biosun.cn
www.biosun.cn 

Feedback

If you would like to receive more detailed information on DIARECT products, please follow this link to our contact and order form.

Contact Details

  • Address: Bötzinger Straße 29 B, 79111 Freiburg, Germany

  • Phone: +49 761 479 79-0

  • Fax: +49 761 479 79-29

  • Email: info@diarect.com

DIARECT AG is a DIN EN ISO 9001 and 13485 certified company.
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