Colorimetric Substrates

The right substrate can make a substantial contribution to the overall sensitivity of your assay. The following BioFX® substrates with different kinetic formulations give you the freedom to choose the substrate that optimizes the dynamic range of your immunoassay without compromising assay sensitivity.

TMB and ABTS HRP Microwell Substrates

Product Code Quantity Product Name Downloads Data Sheet

TMBW-1000-01

1000 mL

TMB One Component HRP Microwell Substrate

TMB One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

TMBS-1000-01

1000 mL

TMB Super Sensitive One Component HRP Microwell Substrate

TMB Super Sensitive One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

TMSK-1000-01

1000 mL

TMB Slow Kinetic One Component HRP Microwell Substrate

TMB Slow Kinetic One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

TMBC-1000-01

1000 mL

TMB Conductivity One Component HRP Microwell Substrate

TMB Conductivity One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

TTMB-1000-01

1000 mL

TMB Super Slow One Component HRP Microwell Substrate

TMB Super Slow One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

TMDS-1000-01

1000 mL

TMB Double Slow One Component HRP Microwell Substrate

TMB Double Slow One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO   

TMBX-1000-01

1000 mL

TMB Extended Range HRP Microwell Substrate

TMB Extended Range HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution.
INFO    

ABTS-1000-01

1000 mL

ABTS One Component HRP Microwell Substrate - Extended Shelf Life

ABTS One Component HRP Microwell Substrate – Extended Shelf Life contains 2.2’-azino-di[3-ethylbenzthiazoline sulfonate (6)] in a mild acidic buffer. The substrate is supplied as a one component ready to use solution.
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TMB HRP Membrane Substrates

Product Code Quantity Product Name Downloads Data Sheet

TMBM-1000-01

1000 mL

TMB One Component HRP Membrane Substrate

TMB One Component HRP Membrane Substrate is a ready to use solution containing 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. This substrate is not recommended for microwell or immunohistochemical applications.
INFO    

ESPM-1000-01

1000 mL

TMB Enhanced One Component HRP Membrane Substrate

TMB Enhanced One Component HRP Membrane Substrate is a ready to use solution containing 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. This enhanced formulation extends the dynamic range of detection, especially at higher concentrations of peroxidase where other substrates may exhibit loss of signal due to washout effects.
INFO    
   

AP Microwell and Membrane Substrates

Product Code Quantity Product Name Downloads Data Sheet

PNPS-1000-01

1000 mL

AP-Yellow One Component Microwell Substrate (pNPP) with Stabilizing Pellets

AP-Yellow One Component Microwell Substrate (pNPP) with Stabilizing Pellets contains p-nitrophenylphosphate (p-NPP) and Stabilizing Pellets (Product Code STAB). The substrate is supplied as a one component ready to use solution.
INFO    

BCIB-1000-01

1000 mL

BCIP/NBT Blue One Component AP Membrane Substrate

BCIP/NBT Blue One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium. The substrate is a ready to use solution.

INFO    

BCID-1000-01

1000 mL

BCIP/NBT Purple One Component AP Membrane Substrate

BCIP/NBT Purple One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium. The substrate is a ready to use solution.
INFO  
 
  • MSDS
  • Product Insert