Substrates FAQ

Frequently Asked Questions about Colorimetric Substrates

What are the shelf life and storage recommendations for SurModics TMB Substrates?
To get an overview of all the TMB Substrates, please click here.

Can the TMB Substrates be diluted?
Dilution of the substrates is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.

What solvent is used in TMB Substrates?
A proprietary non-toxic, non-hazardous, non-carcinogenic solvent is used in our TMB Substrates.

Why do you offer multiple TMB substrates for microwell applications?

All of our microwell TMB substrates have the same lower detection limit. The different formulations provide differing rates of color formation at all analyte levels. This provides flexibility in the timing of your assay.

What wash is best suited for membrane applications?
We typically recommend washing membrane with 0.2 M Tris buffer, pH 7.0 to 7.5 prior to substrate use.

How do you stop the reaction of Product Codes TMBM and ESPM?
To stop the reaction, rinse the membrane with reagent quality water.

Is it recommended to dilute Product Codes TMBM and ESPM?
Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.

Can the stabilizing pellets be removed from Product Code PNPS?
The removal of stabilizing pellets is not recommended.

At what range is the Product Code PNPS read?

Product Code PNPS can be read in the range of 405 nm to 420 nm.

How do you stop the reaction of Product Codes BCIB and BCID?
To stop the reaction, rinse the membrane with reagent quality water.

Can Product Codes BCIB or BCID be used for immunohistochemistry applications?
These products are not recommended for immunohistochemistry applications.